We found that the blastodisc of the embryo, which contains a thick actin cortex (~13 μm), is loaded with cortical Myo1. While Myo1 isoforms appears to be important for both the formation and the maintenance of cleavage furrows, Myo2 is required only for the formation of furrows. Inhibition of Myo1 (by PClP) and Myo2 (by Blebbistatin) lead to arrest in cell division. Our results indicate a unique involvement of Myo1 in early development of Zebrafish embryos. We have studied the effect of Myo1 inhibitor PClP in 1-8 cell Zebrafish embryos. Myosin-1 (Myo1) represents a mechanical link between the membrane and actin-cytoskeleton in animal cells.
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